Speakers

This speech presents an innovative approach to enhance electron transfer-based therapies by coupling electroactive liposome membranes with nanoparticles for cancer treatment and cellular imaging applications. Leveraging the extracellular electron transfer (EET) capabilities of Shewanella oneidensis MR-1, membrane-integrated liposomes (MILs) rich in c-type cytochromes (c-Cyts) were developed using a liposome fusion-induced membrane exchange method. These electroactive MILs were employed to boost the efficacy of nanoparticles, such as TiO₂ and gold nanoparticles (Au NPs). First, TiO₂@MIL nanoparticles demonstrated enhanced superoxide anion production and hydroxide radical generation under low-dose X-ray irradiation, indicating significant potential for radiocatalytic cancer treatments. Second, Au@MIL constructs disrupted the redox balance in cancer cells by serving as electron sinks, leading to oxidative stress, lipid oxidation, and apoptosis under hypoxic conditions without relying on iron-mediated pathways. Together, these findings demonstrate the versatility of electroactive MILs in enhancing nanoparticle-based therapies, offering a promising avenue for advancing cancer treatment and biological applications through modulated electron transfer.